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Table 2 Thermodynamic constants for the binding of MIP NPs to different analytes measured by Isothermal Titration Calorimetry (ITC)

From: Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25

NP Ligand Kd (nM) n ΔH (kJ/mol) ΔG (kJ/mol)
02 MIP32 DTHFPI 6 ± 3 0.4 ± 0.1 −261 ± 18 −47 ± 2
02 MIP200 DTHFPI 7 ± 1 0.4 ± 0.1 −128 ± 25 −46 ± 1
05 MIP32 DTHFPI 3 ± 1 0.4 ± 0.1 −177 ± 14 −49 ± 1
05 MIP200 DTHFPI 5 ± 3 0.5 ± 0.1 −131 ± 4 −48 ± 2
05 MIP200 Hepcidin-25 13 ± 2 0.3 ± 0.1 −463 ± 46 −51 ± 9
05 MIP200 Hepcidin-20
05 MIP200 THFDPI 19 ± 5 0.5 ± 0.1 −395 ± 27 −37 ± 2
05 MIP200 NR10
02 NIP DTHFPI
05 NIP DTHFPI
05 NIP Hepcidin-25
  1. Experiments were performed by titrating 1.2 µM NPs with 4 µM ligand: dissociation constant (Kd), binding stoichiometry (n), enthalpy and Gibbs free energy variation. The heat contribution of control NPs was subtracted from MIP NPs, thus the resulting interactions are primarily driven by specific binding sites. Thermograms in Additional file 1