Table 2 Thermodynamic constants for the binding of MIP NPs to different analytes measured by Isothermal Titration Calorimetry (ITC)
NP | Ligand | Kd (nM) | n | ΔH (kJ/mol) | ΔG (kJ/mol) |
|---|---|---|---|---|---|
02 MIP32 | DTHFPI | 6 ± 3 | 0.4 ± 0.1 | −261 ± 18 | −47 ± 2 |
02 MIP200 | DTHFPI | 7 ± 1 | 0.4 ± 0.1 | −128 ± 25 | −46 ± 1 |
05 MIP32 | DTHFPI | 3 ± 1 | 0.4 ± 0.1 | −177 ± 14 | −49 ± 1 |
05 MIP200 | DTHFPI | 5 ± 3 | 0.5 ± 0.1 | −131 ± 4 | −48 ± 2 |
05 MIP200 | Hepcidin-25 | 13 ± 2 | 0.3 ± 0.1 | −463 ± 46 | −51 ± 9 |
05 MIP200 | Hepcidin-20 | – | – | – | – |
05 MIP200 | THFDPI | 19 ± 5 | 0.5 ± 0.1 | −395 ± 27 | −37 ± 2 |
05 MIP200 | NR10 | – | – | – | – |
02 NIP | DTHFPI | – | – | – | – |
05 NIP | DTHFPI | – | – | – | – |
05 NIP | Hepcidin-25 | – | – | – | – |