Fig. 2

Comparison between free and encapsulated CYP against protection from protease inactivation and acidic pH stability. a Residual CYP activity after incubation of P22-CYP and free CYP with 10 U of trypsin per mg of protein during 1 and 20 h. b Residual CYP activity after incubation of P22-CYP and free CYP in pH 5 and pH 6 for 1 h. CYP activity was measured at 100 mM Tris–HCl pH 8 buffer using 2,6-DMP as a substrate and 5 mM of H₂O₂ to initiate the reaction. Treatments were analyzed by one-way ANOVA and multiple comparisons between mean values using Turkey’s test. All values showed to be statistical different (*p < 0.01)