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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Design of a VLP-nanovehicle for CYP450 enzymatic activity delivery

Fig. 3

Cytochrome P450 activity assay in human cervix carcinoma cell line (HeLa). Staining with DAPI show nuclei of HeLa cells labeled as “n”, panels a and d. Endogenous CYP activity over BFC reagent was visualized in HeLa cells as observed in panel b. CYP activity of transfected VLPs-CYP nanoparticles in HeLa cells is shown in panel e. Overlay of DAPI and BFC localize the CYP activity in the cytoplasm of HeLa cells (white arrows), panels c and f. Scale bar represents 20 μm. Cells were visualized with a ×63 (DIC), 1.4 N.A. planapochromatic oil immersion objective

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