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Table 1 Proton and potassium ions fluxes across the membrane of E. hirae ATCC9790 at the presence of Ag nanoparticles and/or 0.1 mM DCCD

From: Effects of various heavy metal nanoparticles on Enterococcus hirae and Escherichia coli growth and proton-coupled membrane transport

Assay conditionsa

Ion fluxes (mM/min/1010 cells)b

Total

DCCD-sensitivec

H+

K+

H+

K+

H+/K+

Control (no additions)

1.32 ± 0.017

0.90 ± 0.0074

0.70 ± 0.019

pd < 0.03

0.31 ± 0.009

p < 0.05

2.2

1:200

2.65 ± 0.007

2.50 ± 0.018

0.85 ± 0.0069

0.55 ± 0.02

0.9

 

p < 0.05

p < 0.05

p < 0.02

p < 0.05

 

1:500

1.80 ± 0.0085

1.66 ± 0.0081

1.00 ± 0.0075

0.70 ± 0.018

1.3

 

p < 0.05

p < 0.05

p < 0.05

p < 0.05

 
  1. aThe bacteria were washed and transferred in Tris–phosphate buffer (pH 8.0) containing 0.4 mM MgSO4, 1 mM NaCl, 1 mM KCl; 20 mM glucose was added
  2. bCalculated per 1010 cells/ml
  3. cThe difference between fluxes in parallel experiments in the absence and presence of 0.1 mM DCCD
  4. dP was calculated for difference between the values of experimental samples and appropriate control
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Journal of Nanobiotechnology

ISSN: 1477-3155

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