Fig. 2
Assessment of cytotoxic and genotoxic stress in cultured hMSCs. a Representative flow cytometry histograms of UT- and SiO2-NPs -treated hMSCs at t0: forward scatter (FS) in function of propidium iodide fluorescence (PI) allows discriminating living cells (bottom population) from dead cells (top population). b Quantification of hMSCs viability during in vitro culture, represented as percentage variation respect to UT cells at the same time point. c, d Representative immunofluorescence experiments for γH2AX in UT- and SiO2-NPs -treated hMSCs at t0. Magnification 63×, scale bar 20 µm. e Quantification of percentage positive cells for nuclear γH2AX foci during culture