Fig. 3

Phenotypic Assays on Biofilm inhibition by AgNPs. a Different AgNPs concentrations in two-fold serial dilutions were added to C. albicans cells. The IC₅₀ values for inhibition of biofilm formation were calculated as AgNPs 0.089 ppm (b) AgNPs was also added to preformed (24 h) biofilms. The IC₅₀ values for pre-formed biofilm reduction were calculated as AgNPs 0.48 ppm. All experiments were performed in duplicate on three separate occasions. Results represent the means ± standard deviations (SD). c The cytotoxic concentration 50 % (CC₅₀) of AgNPs was measured in HEPG2 cell line. The experiments were performed in triplicate, and the results are presented as the mean ± SE. The CC₅₀ values for cell toxicity were calculated as AgNPs 7.08 ppm