Fig. 3
Accumulation of ceroid lipopigment in LINCL fibroblasts treated with polystyrene nanoparticles. a Confocal microscopy analyses of TFEB (red) and ceroid lipopigment (green) in LINCL fibroblasts treated with PS, PS-COOH or PS-NH2 (25 µg/mL) for 3 days and evaluated by detecting green autofluorescence and binding of anti-TFEB antibody, respectively. UT untreated. The scale bar is 20 μm. b Quantification of ceroid lipopigment fluorescence intensity calculated as described in the “Methods”. Data are reported as mean ± SD (n = 15; *p < 0.01)