Fig. 5
Colocalization of LC3 and LAMP-2 in fibroblasts treated with polystyrene nanoparticles. a Quantification of LC3-LAMP-2 colocalization in fibroblasts treated with polystyrene nanoparticles (50 nm; 25 µg/mL) calculated as described in the “Methods”. UT untreated. Data are reported as mean ± SD (n = 15; *p < 0.01). b–e Representative confocal microscopy images of LC3 (red) and LAMP-2 (blue) in b untreated fibroblasts (UT) and in fibroblasts treated with c PS (25 µg/mL), d PS-COOH (25 µg/mL), e PS-NH2 (25 µg/mL) after 24, 48, and 72 h evaluated by detecting binding of anti-LC3 antibody and binding of anti-LAMP-2 antibody, respectively. Colocalization of LC3 (red) and LAMP-2 (blue) is shown in merged images (purple). The scale bar is 20 μm