Fig. 2
MW-CNTs affect astrocyte morphology as shown by scanning electron microscopy. a, b show primary cultured cortical astrocytes on PDL coverslips. c, d show astrocytes on MW-CNT-50 coverslips with much rounder cells. e, f show astrocytes on MW-CNT-1000, cell growth is similar to that seen on PDL. g shows roundness parameter graph. Astrocytes on MW-CNT-50 (n = 8) showed higher roundness factor compared to astrocytes on PDL (n = 10) and MW-CNT-1000 (n = 7). Roundness factor formula is 4π × [area (μm2)/[perimeter (μm2)]. h graph shows MW-CNTs (MW-CNT-50; n = 8, MW-CNT-1000; n = 7) make more cell–cell interactions, with many cell processes, compared to astrocytes on PDL coverslips (n = 10); (*p < 0.05), (**p < 0.01). Significant effects were determined by a one-way ANOVA analysis of variance