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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Engineered atherosclerosis-specific zinc ferrite nanocomplex-based MRI contrast agents

Fig. 1

Characterization of zinc doped ferrite nanoparticles and their nanocomplexes. a Transmission electron microscopy (TEM) image confirmed formation of uniformly sized spherical zinc-doped ferrite nanoparticles (Zn0.4Fe2.6O4) with an average diameter of 24 ± 2.8 nm. b X-ray differactogram revealed the formation of crystallographic spinel structure of zinc-doped ferrite nanoparticles. c TEM image revealing the formation of Hsp-70 Lf- ZF nanocomplex. d DLS analysis of Hsp-70 Lf-ZF nanoparticles revealed bimodal hydrodynamic size distribution with the prominent primary peak at 435.4 nm and a secondary peak at 55.6 nm with a polydispersity index of 0.876. e TEM image revealed the formation of Hsp-70 Ch-Lf-ZF nanocomplex. f DLS analysis revealed the hydrodynamic diameter of 275 nm with a PDI of 0.865. g TEM image revealing the formation of Hsp-70 Lf-PEG-ZF nanocomplex and a highly magnified image (inset). h Dynamic light scattering (DLS) revealed the hydrodynamic diameter of 224.8 nm with a polydispersity index of 0.398. i Sodium dodecyl sulfate—polyacrylamide gel electrophoresis (SDS-PAGE) confirming lactoferrin loading of the nanocomplexes. From left to right, standard protein ladder, pure bovine lactoferrin (Lf), ZF nanoparticles, Hsp-70 Lf-ZF, Hsp-70 Lf-PEG-ZF and Hsp-70 Ch-Lf-ZF nanocomplexes. Nanocomplex lactoferrin loading was confirmed with specific band detection at 75 kDa

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