Fig. 3

The levels of cytokines IL-4 (a), IL-5 (b) and IL-12 (c) in supernatants of OVA-stimulated mouse splenocytes taken after 3-rd sensitization and incubated with OVA for 72 h (quantified by ELISA). Relative expression of IFN-γ mRNA (d) in mouse splenocytes taken after 3-rd sensitization and incubated with OVA for 72 h (quantified by real-time PCR). The results are presented as a mean concentration (mean ± SE, n = 8 for each). Designations of columns in the diagram are the same as those in Fig. 2; *p < 0.05