Fig. 4

In vivo skin permeation of LPPC/DiI or cream/DiI after treatment in BT474 tumor-bearing mice. a BT474 tumor-bearing mice were applied the cream and LPPC containing DiI fluorescent dye (red fluorescence) to tumor area once. The tumor sample covered with skin were collected and fixed at different time periods. All fixed tumor samples were vertically cut and the fluorescent intensity was evaluated for permeation efficiency by fluorescent microscope. Hematoxylin & Eosin staining in above panels showed the structure of skin to tumor. Below panel showed the drug accumulation by skin permeation. b The DiI accumulation in tumor area after treatment with LPPC/DiI or cream/DiI. The fluorescent score were identified as 0–3 for <5, 5–25, 26–50 and >50 %, respectively