Fig. 3
From: Bioluminescent magnetic nanoparticles as potential imaging agents for mammalian spermatozoa
Bioluminescence imaging (BLI) of CA-MNP, Luc + MNPs, and Luc only. Spermatozoa were labeled in PBS solution and then centrifuged. Sperm pellets from the Luc + MNPs, and Luc samples, and their corresponding supernatants (sCA-MNP, sLuc + MNP), were separately imaged after supplementation with coelenterazine. (Note that measurements were performed on 1.5 mL centrifuge tubes with ~50 µL of sample.) a Sample images with light (BLI) signal intensities shown as a gradient color scale. b Quantified BLI signals (mean ± sem). Asterisk indicates significant difference from CA-Luc Control (ANOVA-2, P < 0.05, N = 4 replicates)