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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cells

Fig. 1

Schematic representation of nanosensor. a Ligand free form of LAOBP from S. typhimurium showing the N-terminus and C-terminus, b linear representation of the nanosensor construct and c schematic representation of the lysine induced conformational change in the LAOBP. With the result of binding of lysine to LAOBP, CFP and YFP come closer to each other. Emission of CFP at this stage excites the YFP. Ratio of YFP/CFP emission (FRET ratio) changed as compared to unbound stage

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Journal of Nanobiotechnology

ISSN: 1477-3155

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