Fig. 4From: Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cellspH stability of the FRET signal of FLIPK. YFP/CFP emission ratios were measured in PBS buffer with different pH, in the absence (blue) and in the presence (red) of 1 mM of lysine. Stability increases with pH while the sensor stabilizes above pH 7.0. Concentration of sensor protein was 0.16 mg/ml. Values are means of three independent replicates. Vertical bars indicate the standard errorBack to article page