Skip to main content
Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cells

Fig. 6

In vitro ligand dependent FRET ratio change of FLIPK in the presence of l-lysine. Affinity mutants Y14A, R77L, F52A, D161I and S72A were developed. Mutations at position 14 tyrosine substituted by alanine (Y14A), arginine 77 to leucine (R77L), phenylalanine 52 to alanine (F52A), and aspartic acid 161 to isoleucine (D161I) and serine at 72 position by alanine (S72A). Concentration of sensor protein was 0.24 mg/ml. Values are means of three independent replications. Vertical bars indicate standard error

Back to article page