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Table 1 FLIPK affinity mutants

From: Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cells

Sensor namea

Sequences

K d (µM)

Dynamic rangeb (µM)

FLIPK-97µ

Wild type

97

14–980

FLIPK-30µ

S72A

30

4.0–208

FLIPK-142µ

Y14A

142

20–1000

FLIPK-306µ

R77L

306

65–1500

FLIPK-2 m

D161I

2000

1000–4500

FLIPK-14 µ

F52A

14

2.0–150

  1. Binding constants were determined in vitro
  2. aNumber next to the mutant name stands for the Kd determined for the sensor variants
  3. b Effective quantification range between 10 and 90 % saturation of the sensor
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Journal of Nanobiotechnology

ISSN: 1477-3155

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