Fig. 1

Characterization of a 3D cell model mimicking a metastatic lymph node. a A549 lung cancer cells and Jurkat lymphocytes were co-cultured in polystyrene scaffolds and observed as a tumor-like mass by optical microscopy. b Internal structure and architecture of the 3D co-culture was verified by scanning electron microscopy (SEM) at 500× magnification. c The presence of lymphocytes embedded in the culture (red: lymphocytes stained with DiI) was confirmed by confocal laser scanning microscopy (CLSM). Cell nuclei of both tumor cells and lymphocytes were counterstained with DAPI, blue channel