Fig. 2

Cellular uptake studies in A549 lung adenocarcinoma cells growing in 2D monolayers. Cells were incubated for 24 h with a DiD-loaded and b DiD-DCX-loaded PGA-PEG NCs (green channel). Cell nuclei were counterstained with DAPI (blue channel). Accumulation of nanocapsules carrying DiD and docetaxel in the cells produced a cytotoxic effect characterized by nuclei fragmentation. Insets show the boxed areas at a higher magnification