Fig. 6

Toxicity profile of void liposomes. a The toxicity of void liposomes was examined in ten pancreatic cancer cell lines as well as non-malignant HPNE cells. These cells were exposed to increasing doses of void liposomes for 72 h and growth inhibition was determined using MTS assays. b The hemolytic activity of void or EF24-loaded liposomes on erythrocytes (RBCs) was evaluated using ex vivo RBC hemolysis assays. c In vivo toxicity studies were performed by systemic administration of void liposomes, Lipo-EF24 (both at 10 mg/kg i.v. thrice weekly for a total of 3 weeks) or PBS as mock treatment control to CD1 wildtype mice. Body weights in the three respective treatment arms were assessed on a weekly basis and no significant differences in mean body weights were observed during the course of experiment. d Histopathological assessment of major organs obtained from these mice did not show any discernible abnormalities or other evidence of toxicity. Representative HE sections of major organ sites obtained from mice treated with void liposomes (VL) or Lipo-EF24 (EL) are shown