Fig. 8

In vivo therapeutic effect of Lipo-EF24 on MIAPaCa xenograft tumor growth. a Growth of subcutaneous MIAPaCa xenografts was significantly inhibited in nude mice after treatment with Lipo-EF24 and gemcitabine in combination as shown by measured tumor volumes (n = 8) for each treatment arm after 3 weeks. Lipo-EF24 or gemcitabine used as single agents did not show any significant effect when compared to void liposomes as control. b Tumor weights measured at the end of treatment also showed significant reduction in the combination treatment arm as compared to either single agents or void liposomal control. c During the course of treatment, no difference in the mean body weight of mice was observed between the respective treatment arms. d Lipo-EF24 in combination with gemcitabine suppressed NF-kappaB activation in MIAPaCa xenografts, as demonstrated by marked inhibition of the phosphorylated form of its inhibitor protein I-kappa-B-alpha as well as NF-kappaB-p65, determined by western blot analysis of frozen tumor tissues