Fig. 3
From: Gd-nanoparticles functionalization with specific peptides for ß-amyloid plaques targeting
Spectrofluometry measurement of PIB binding to Aβ (1–42) fibrils or V30M-TTR fibrils following partition experiments (λex = 350 nm and λem = 420 nm). a 2 µM of PIB was added to 20 µM of amyloid fibrils (dotted line) and after 60 min., a pellet with fibrils and PIB bound (double lines) was separated from a supernatant with PIB alone (solid line). (b, c) Binding plots by fluorescence response at 420 nm of PIB bound on Aβ(1–42) fibrils (B) or V30M-TTR fibrils (c) increasing concentration of PIB, fitted as Michaelis model (1:1)