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Table 1 List of primary antibodies, secondary antibodies and nucleic acid stain used in study

From: Impact of degradable nanowires on long-term brain tissue responses

Name

Characteristics

Host

Working dilution

Source

ED1 (CD68)

Activated microglia/macrophages

Mouse

1:250

Cat. Nr. MCA341R, AbD Serotec, UK

GFAP

Glial fibrillary acidic protein

Rabbit

1:5000

Cat. Nr. Z0334, Dako, Denmark

NeuN

Neuronal nuclei, neuronal marker

Mouse

1:100

Cat. Nr. MAB377, Millipore, USA

Alexa Fluor 594

Goat anti-rabbit

Goat

1:500

Cat. Nr. A11005, Invitrogen, USA

Alexa Fluor 488

Goat anti-mouse

Goat

1:500

Cat. Nr. A11001, Invitrogen, USA

DAPI

Nucleic acid stain (4′,6-diamidino-2-phenylindole)

1:1000

Cat. Nr. D3571, Invitrogen, USA