Fig. 4

Induction of ScaA specific immune responses. a Antibody responses observed in the immunized mice. Mice were immunized with ZNP, ZBP-ScaA, ZNP/ScaA, NP/ZBP-ScaA, or Alum/ZBP-ScaA (n = 3/group) twice and the levels of anti-ScaA IgG₁ and IgG_2C in the sera were measured by ELISA at 1 week after immunization. Antibody titer was assessed up to 102,400. b IFN-γ-positive CD4⁺ or CD8⁺ T cells in splenocytes stimulated with ScaA antigen were detected at 1 week after second immunization as in a. Representative dot blots obtained by FACS analysis were presented. c Average percentile of three independent experiments for IFN-γ-positive CD4⁺ or CD8⁺ T cells in splenocytes from immunized mice were presented. Error bars mean ± SD