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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A critical review of the current knowledge regarding the biological impact of nanocellulose

Fig. 3

Reprinted with permission from Endes et al. [79] © 2015 American Chemical Society

Length dependent clearance of CNCs by macrophages. Confocal laser scanning microscopy images of the triple-cell co-culture model exposed to 0.56 ± 0.25 μg/cm2 rhodamine-labeled CNCs isolated from cotton (green ad) or 0.67 ± 0.09 μg/cm2 CNCs isolated from tunicates (eh) via the ALICE system. Co-cultures were either immediately fixed (a, e) or after 1 (b, f), 24 (c, g), or 48 h (d, h) post exposure and stained for cytoskeleton (red) and nuclei (cyan). Images are presented as surface rendering (top), xz-projection of the z-stacks (middle), or twofold optical zoom (bottom). Boxes indicate digitally enlarged (×2) areas. Arrow shows fiber-F-actin interactions. Scale bars 30 μm.

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