Table 2 Binding constants (KD) of t-PA-peptide-1 and its derivates with galectins Gal-1, Gal-3 and Gal-4
T-PA peptide | Label | Modification | Galectin | KD [µM] | Fold diff. |
|---|---|---|---|---|---|
rh t-PA protein | N-Fluo. | None = (Asn16) | Gal-1 | 4.92 | 1 |
Peptide-1(pep-1) | N-Fluo. | None | Gal-1 | 141.1 | −28.7 |
t-PA-pep-1 | C-Fluo. | None | Gal-1 | Â | Â |
t-PA-pep-1gal | C-TAMRA | Ser16-α d-galactose | Gal-1 | 0.66 | 7.5 |
t-PA-pep-1lac | N-Fluo. | Ser16-β d-lactose | Gal-1 | 0.286 | 17 |
t-PA-pep-1lac | C-Fluo. | Ser16-β d-lactose | Gal-1 | 0.20 | 25 |
rh t-PA protein | N-Fluo. | None = (Asn16) | Gal-3 | 7.68 | 1 |
t-PA-pep-1 | N-Fluo. | None | Gal-3 | 272.0 | −35.4 |
t-PA-pep-1gal | C-TAMRA | Ser16b-α d-galactose | Gal-3 | 39.1 | −5.1 |
t-PA-pep-1lac | C-Fluo. | Ser16b-β d-lactose | Gal-3 | 0.73 | 10.4 |
rh t-PA protein | N-Fluo. | none = (Asn16) | Gal-4 | 51.2 | 1 |
t-PA-pep-1 | N-Fluo. | none | Gal-4 | 34.0 | 1.5 |
t-PA-pep-1gal | C-TAMRA | Ser16-β d-galactose | Gal-4 | 211 | −4.2 |
t-PA-pep-1lac | C-Fluo. | Ser16-β d-lactose | Gal-4 | 12.7 | 4.03 |