Fig. 4

Apoptotic assay of HepG2/ADM cells induced by GPMQNs. A Flow cytometric measurement of cellular apoptosis of resistant HepG2/ADM cells treated with various reagents; a untreated cells, b treatment of cells with 1 mg L⁻¹ GPMQNs, c treatment of cells with 2 mg L⁻¹ GPMQNs, d transfected cells with 4 mg L⁻¹ GPMQNs, e transfected cells with 8 mg L⁻¹ GPMQNs, f treatment of cells with 10 mg L⁻¹ GPMQNs, g histograms of apoptotic rate of HepG2/ADM after various treatments as shown in (a, b, c, d, e, f). B DNA fragmentation in resistant HepG2/ADM cells after different treatments; 1 untreated cells, 2 treatment of cells with 10 mg L⁻¹ GPMQNs without loading miR-122, 3 transfected cells with miR-122 (1 mg L⁻¹, same concentration as loaded on GPMQNs), 4 treatment of cells with 10 mg L⁻¹ GPMQNs. C Western blot analysis after various treatments; 1 untreated cells, 2 treatment of cells with 10 mg L⁻¹ GPMQNs without loading miR-122, 3 transfected cells with miR-122 (1 mg L⁻¹, same concentration as loaded on GPMQNs), 4 treatment of cells with 10 mg L⁻¹ GPMQNs, (*P < 0.05 compared to the control group)