Fig. 4From: Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell linesQuantification of the cellular uptake of PHBV nanoparticles: energy dependency. To evaluate energy dependency in the cellular uptake of NPs, HeLa (a) and SKOV-3 cells (b) were incubated with 100 µg/mL PHBV-RN under different conditions (37, 4 °C, sodium azide). Mean fluorescence intensity was determined through flow cytometry (n = 3). One-way ANOVA and Bonferroni statistical test were used (**p < 0.01, ***p < 0.001, ****p < 0.0001)Back to article page