Fig. 9

Analysis of proteins involved in cellular uptake of PHBV nanoparticles. HeLa and SKOV-3 cells were subjected to reducible biotin assay to determine possible membrane proteins involved in nanoparticles uptake. 40 µg of proteins from cell lysate were resolved through SDS-PAGE, then transferred to PDVF membrane and finally, incubated with streptavidin-HRP (1:1000). β-actin (47 kDa) was used as loading control