Fig. 2

Characterization of the DENV3E recombinant protein and the MWNT functionalization process. a Western blot analysis: column 1 was loaded with an irrelevant recombinant protein (37 KDa) and probed with its respective specific antibody as an external positive control. Column 2 was loaded with an eluate from non-transformed bacteria, as negative control, and probed with monoclonal anti-DENV antibodies. Columns 3 and 4 were loaded with eluates from recombinant DENV3E (~50 kDa) producing bacteria and probed with either monoclonal anti-DENV antibodies or polyclonal anti-HIS antibodies, respectively. b After functionalization, the MWNT-DENV3E solution was washed 3 times (1st, 2nd and 3rd) to remove unbound proteins and the flow through was analyzed by SDS-PAGE. Three different MWNT to protein ratios (w/w using 1 µg of MWNT) were tested (1/1, 1/3 and 1/5). c TEM of carboxylated MWNT. d TEM of MWNT-DENV3E, black arrow points to typical deposition of amorphous material on the protein-functionalized MWNT surface. Scale bars are represented in both c, d