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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Toxicity of nano- and ionic silver to embryonic stem cells: a comparative toxicogenomic study

Fig. 3

Global gene expression responses of AgNPs and Ag+ in differentiating mESCs. a Schematic representation of the experimental procedure. The slate blue arrow covers the embryoid body (EB) formation stage. Hanging drops were set up on day 0 and EBs formed on day 3. ESC differentiation started from day 3 onwards. AgNP or Ag+ exposure is shown by the maroon arrow, which lasted for 24 h (from day 3 to day 4). b Principal component analysis (PCA) using 16,510 filtered genes to cluster samples based on their similarities or dissimilarities. The three axes represent the first three principal components identified by the analysis. c Hierarchical cluster analysis using 459 unique DEGs to cluster samples based on their similarities or dissimilarities. The clustering was performed through Ward’s minimum variance linkage on normalized expression data which are in log2 scale and color coded as shown in the scheme at the top right corner. The tree on the right of the image shows clusters of genes (names not shown), while the tree on the top of the image shows clusters of samples. Samples labeled as Ag+ were treated with 5.0 µg/ml of Ag+. Those treated with 1.0 µg/ml Ag+ were omitted from the analysis

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