Table 1 Optimization of working concentration
Coating antibody | 10 μg/mL | 5 μg/mL | 1 μg/mL | 0.1 μg/mL | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CAg (ng/mL) | 0 | 1 | 50 | 0 | 1 | 50 | 0 | 1 | 50 | 0 | 1 | 50 | |
D | 1:100 | 33,506 | 39,216 | 614,441 | 30,142 | 35,891 | 640,496 | 27,993 | 41,092 | 635,737 | 32,337 | 29,902 | 40,606 |
1:200 | 27,232 | 36,745 | 495,871 | 29,246 | 36,645 | 619,561 | 25,237 | 32,604 | 507,215 | 28,922 | 27,300 | 36,226 | |
1:300 | 26,143 | 31,833 | 344,716 | 27,453 | 34,398 | 427,663 | 22,174 | 33,261 | 388,885 | 27,790 | 27,253 | 31,046 | |
1:400 | 25,002 | 31,137 | 240,503 | 27,130 | 30,330 | 247,904 | 22,373 | 32,398 | 192,264 | 28,985 | 28,918 | 29,168 | |