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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A mechanistic investigation exploring the differential transfection efficiencies between the easy-to-transfect SK-BR3 and difficult-to-transfect CT26 cell lines

Fig. 3

Effects of chloroquine on endosomal escape in SK-BR3 and CT26 cells. GFP reporter gene plasmids were delivered into a SK-BR3 and b CT26 cells either complexed to AuPAMAM or PEI. Cells were treated with 1000 μM chloroquine, and allowed to incubate for 3 h. Enhancement of endosomal escape and overall transfection efficiency by chloroquine was then visualized by fluorescence microscopy. Images shown are fluorescent images overlaid on transmission images

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