Fig. 4

Subcellular trafficking of Cy5-labeled AuPAMAM/DNA complexes in SK-BR3 cells. a The intracellular trafficking of Cy5-labeled GFP reporter gene plasmid DNA (shown in red) was observed in SK-BR3 cells 1-, 4-, 24-, and 48-h post-transfection via confocal microscopy. Column (a) depicts 60× magnification and column (b) depicts Nyquist zoom of the corresponding images in column (a). Column (c) exhibits overlays of the fluorescent channels with the transmission channel for the corresponding images in column (b). Scale bar is (a) 20 μm and (b, c) 10 μm