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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Targeted siRNA delivery reduces nitric oxide mediated cell death after spinal cord injury

Fig. 3

Knockdown of iNOS mRNA in activated M1 macrophages. All groups were challenged with 100 ng/ml LPS and 20 ng/ml IFNγ for 12 h. Activated macrophages without siRNA silencing were indicated as LPS + IFNγ. siRNA* w/TR for 48 h denotes cells transfected with siRNA and a lipid transfection reagent for 48 h. Stimulated cells were also exposed to siRNA w/TR for 4 h (siRNA* w/TR for 4 h). Different antibody conjugated chitosan NPs ranging from 10 to 30% Ab level were also screened for efficacy in lowering iNOS expression (indicated as 10, 20, 30% Ab-siRNA*-chitosan NPs, respectively). siRNA conjugated chitosan NPs without the antibody moiety are labeled as siRNA*-chitosan NPs. Chitosan NPs coupled to scrambled siRNA sequences [siRNA (N)] either with or without antibody conjugation are denoted with grey bars. **p ≤ 0.01; ***p ≤ 0.001 vs LPS + IFNγ

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