Fig. 3

Biogenic SeNPs induce ROS-mediated cytotoxicity in PC-3 cancer cells. 24 h cultured PC-3 cancer cells were treated with SeNPs at a concentration of 2 µg Se/ml for 12 h. Mitochondrial ROS were visualized using dihydrorhodamine 123 at 60× oil immersion under the confocal microscope with 488 nm argon laser. ROS-induced cleavage of dihydrorhodamine 123 produces a fluorescent molecule, rhodamine 123, which was evident in the form of greenish yellow colored puncta