Fig. 5

Endocytosis of SeNPs induces ROS-mediated cell death. a Cytochalasin D treated and untreated cells cultured in the presence of SeNPs (2 µg Se/ml) for 16 h showed more viability in cytochalasin D treated cells on AnnexinV-PI staining. b Staining with DHR123 also showed insignificant ROS production in cytochalasin D-treated cells. c TEM image of a PC-3 cell, acquired after 12 h treatment with SeNPs, showing the cytoplasmic localization of NPs. All the experiments were performed in triplicate