Fig. 7

a Western blot analysis of necroptosis associated proteins. A significantly elevated level of RIP1 protein was observed after 12 h treatment with SeNP compared to the untreated cells signifies its overexpression or stabilization upon SeNP treatment. However, no change was observed in RIP3 and MLKL protein levels. Similarly, the presence of Nec-1 did not affect expression pattern of any of the protein. b PC-3 cells were cultured in the presence of 2 µg Se/ml SeNPs and/or Nec-1 for 24 h. Cell viability was determined by MTT assay. Significantly more viable cells were observed in wells treated with SeNP along with Nec-1. The results were verified by three repetitions of experiments and each experiment was conducted in triplicate. ***p < 0.001 represents a significant difference in the PC-3 cell viability treated with SeNP or SeNP with Nec-1