Fig. 9
Effect of PAK1 depletion on cell uptake of Rubipy-SiO2 NPs. CaCo-2 cells were transfected with si-ctrl or si-PAK1 and after 48 h the remaining RNA expression was evaluated with real-time PCR (a), protein expression was assessed with western blot (b), the cell uptake of Dextran 10 kDA was measured after 30 min incubation (c) and the cell uptake of Rubipy-SiO2 NPs was measured after 3 h (d) and 6 h (e) exposure by flow cytometry. Statistical significance was assessed by a Student’s t test; *p < 0.05, si-PAK1 silenced cells compared to si-ctrl treated cells