Skip to main content
Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Effects of silica nanoparticle exposure on mitochondrial function during neuronal differentiation

Fig. 3

Oxidative phosphorylation enzymes (OXPHOS) were analyzed to evaluate expression of mitochondrial chain complexes I to V in SH-SY5Y cells exposed to PCL-NPs ([2.6 × 1010 PCL-NPs/ml]) for 24 h before (DIV1, NP DIFF) or after (DIV4, DIFF NP DIFF) differentiation with retinoic acid. Untreated controls, undifferentiated SH-SY5Y cells (CO UNDIFF) and differentiated SH-SY5Y cells (CO DIFF) were performed in parallel. The following complexes or subunits of the complexes were analyzed: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase complex (C)I (CI-NDUFB8); iron-sulfur protein (IP) subunit of succinate dehydrogenase involved in CII (CII-SDHB); component of the ubiquinol-cytochrome c reductase of CIII (CIII-UQCRC2); catalytic subunit of cytochrome c oxidase of CIV (CIV- MTCO1), and mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase) or CV (CV-ATP5A). Representative Western blots are shown in (a). The histograms represent the ratio of each complex normalized to the corresponding actin (loading control) band CI (b), CII (c), CIII (d), CIV (e) and CV (f). Respective protein levels were assessed using digital quantification of immunoblots, and they are presented as relative intensity compared to total protein. The analysis was done using ImageJ. Values are expressed with arbitrary units. Error bars represent the mean + SEM

Back to article page