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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: A versatile papaya mosaic virus (PapMV) vaccine platform based on sortase-mediated antigen coupling

Fig. 4

PapMV-SrtA-T20 induces a T20 specific antibody response. We immunized animals [Female Balb/C mice (5/group)] twice, with a 14-day interval by the intramuscular route with either 30 or 90 µg of PapMV-SrtA-T20, PapMV-SrtA or T20 peptide, to evaluate if coupling of T20 peptides fused to the surface of PapMV nanoparticles enhance the humoral response to the T20 peptide antigen. Blood was taken 14 days following each immunization, and ELISA assays were performed to evaluate levels of anti-T20 total IgG (a) or IgG2a (b) after the first immunization, and anti-T20 total IgG (c) or IgG2A (d) after the booster immunization. ****P < 0.0001 all PapMV-SrtA-T20 groups against PapMV-SrtA alone, or PapMV-SrtA and T20 peptide. ***P < 0.001 between 30 and 90 µg of PapMV-SrtA-T20 total IgG (d27), ****P < 0.0001 total IgG (d13) and **P < 0.01 IgG2a (d13 and d27)

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