Table 1 List of primers and sequences (according to HUGO gene nomenclature; http://www.genenames.org)
Primer name (abbreviated) and NCBI RefSeq | Forward primer sequence and Tm | Reverse primer sequence and Tm | Amplicon size (bp) | References |
|---|---|---|---|---|
18S (NR_003286.2; NT_167214.1) | 5′-AGAAACGGCTACCACATCCA-3′ 56.3 °C | 5′-CACCAGACTTGCCCTCCA-3′ 57.3 °C | 169 | [8] |
ACTB (NM_001101) | 5′-AGAAAATCTGGCACCACACC-3′ 55.6 °C | 5′-TAGCACAGCCTGGATAGCAA-3′ 56.1 °C | 173 | [9] |
GAPDH (NM_002046) | 5′-CGACAGTCAGCCGCATCTT-3′ 57.8 °C | 5′-CCCCATGGTGTCTGAGCG-3′ 58.5 °C | 63 | [8] |
GUSB (NM_000181) | 5′-AGCCAGTTCCTCATCAATGG-3′ 54.9 °C | 5′-GGTAGTGGCTGGTACGGAAA-3′ 56.8 °C | 160 | [8] |
HPRT1 (NM_000194) | 5′-TGACACTGGCAAAACAATGCA-3′ 56.0 °C | 5′-GGTCCTTTTCACCAGCAAGCT-3′ 58.0 °C | 94 | [8] |
HSP90 (NM-007355) | 5′-TCTGGGTATCGGAAAGCAAGCC-3′ 59.4 °C | 5′-GTGCACTTCCTCAGGCATCTTG-3′ 58.4 °C | 80 | [8] |
PPI (NM_021130) | 5′-AGACAAGGTCCCAAAGAC-3′ 51.6 °C | 5′-ACCACCCTGACACATAAA-3′ 50.7 °C | 118 | [8] |
SDH (NM004168) | 5′-TGGGAACAAGAGGGCATCTG-3′ 57.2 °C | 5′-CCACCACTGCATCAAATTCATG-3′ 54.8 °C | 86 | [8] |
TBP (NM_003194) | 5′-TGCACAGGAGCCAAGAGTGAA-3′ 58.9 °C | 5′-CACATCACAGCTCCCCACCA-3′ 59.8 °C | 132 | [8] |
YWHAZ (NM_003406) | 5′-ACTTTTGGTACATTGTGGCTTCAA-3′ 55.5 °C | 5′-CCGCCAGGACAAACCAGTAT-3′ 57.4 °C | 94 | [8] |