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Table 1 List of primers and sequences (according to HUGO gene nomenclature; http://www.genenames.org)

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Primer name (abbreviated) and NCBI RefSeq Forward primer sequence and Tm Reverse primer sequence and Tm Amplicon size (bp) References
18S (NR_003286.2; NT_167214.1) 5′-AGAAACGGCTACCACATCCA-3′
56.3 °C
5′-CACCAGACTTGCCCTCCA-3′
57.3 °C
169 [8]
ACTB (NM_001101) 5′-AGAAAATCTGGCACCACACC-3′
55.6 °C
5′-TAGCACAGCCTGGATAGCAA-3′
56.1 °C
173 [9]
GAPDH (NM_002046) 5′-CGACAGTCAGCCGCATCTT-3′
57.8 °C
5′-CCCCATGGTGTCTGAGCG-3′
58.5 °C
63 [8]
GUSB (NM_000181) 5′-AGCCAGTTCCTCATCAATGG-3′
54.9 °C
5′-GGTAGTGGCTGGTACGGAAA-3′
56.8 °C
160 [8]
HPRT1 (NM_000194) 5′-TGACACTGGCAAAACAATGCA-3′
56.0 °C
5′-GGTCCTTTTCACCAGCAAGCT-3′
58.0 °C
94 [8]
HSP90 (NM-007355) 5′-TCTGGGTATCGGAAAGCAAGCC-3′
59.4 °C
5′-GTGCACTTCCTCAGGCATCTTG-3′
58.4 °C
80 [8]
PPI (NM_021130) 5′-AGACAAGGTCCCAAAGAC-3′
51.6 °C
5′-ACCACCCTGACACATAAA-3′
50.7 °C
118 [8]
SDH (NM004168) 5′-TGGGAACAAGAGGGCATCTG-3′
57.2 °C
5′-CCACCACTGCATCAAATTCATG-3′
54.8 °C
86 [8]
TBP (NM_003194) 5′-TGCACAGGAGCCAAGAGTGAA-3′
58.9 °C
5′-CACATCACAGCTCCCCACCA-3′
59.8 °C
132 [8]
YWHAZ (NM_003406) 5′-ACTTTTGGTACATTGTGGCTTCAA-3′
55.5 °C
5′-CCGCCAGGACAAACCAGTAT-3′
57.4 °C
94 [8]