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TableĀ 3 Summarised results of the universal RNA standard spiked before transcription (part 1), as generated by the NormFinder analyses

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene NormFinder technical REPEAT 1 NormFinder technical REPEAT 2 NormFinder technical REPEAT 3 NormFinder average Manual ranking using the NormFinder average of the reference gene NormFinder global summary of the best genea
18S (0.679) (0.314) (0.131) 0.374 1 SDH (most stable)
2 YWHAZ
3 GUSB
4 TBP
5 HSP90
6 GAPDH
7 HPRT1
8 ACTB
9 18S
10 PPI (least stable)
GUSB and HSP90 (0.121)
GUSB (0.126)
HSP90 (0.136)
ACTB (0.368) (0.212) (0.118) 0.232
GAPDH (0.180) (0.175) (0.099) 0.151
GUSB (0.198) (0.088) (0.037) 0.107
HPRT 1 (0.202) (0.149) (0.169) 0.172
HSP90 (0.094) (0.118) (0.155) 0.122
PPI (0.246) (0.668) (0.260) 0.391
SDH (0.063) (0.078) (0.070) 0.070
TBP (0.095) (0.145) (0.106) 0.115
YWHAZ (0.197) (0.038) (0.029) 0.088
  1. aThe best gene has the lowest stability value