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Table 3 Summarised results of the universal RNA standard spiked before transcription (part 1), as generated by the NormFinder analyses

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene

NormFinder technical REPEAT 1

NormFinder technical REPEAT 2

NormFinder technical REPEAT 3

NormFinder average

Manual ranking using the NormFinder average of the reference gene

NormFinder global summary of the best genea

18S

(0.679)

(0.314)

(0.131)

0.374

1 SDH (most stable)

2 YWHAZ

3 GUSB

4 TBP

5 HSP90

6 GAPDH

7 HPRT1

8 ACTB

9 18S

10 PPI (least stable)

GUSB and HSP90 (0.121)

GUSB (0.126)

HSP90 (0.136)

ACTB

(0.368)

(0.212)

(0.118)

0.232

GAPDH

(0.180)

(0.175)

(0.099)

0.151

GUSB

(0.198)

(0.088)

(0.037)

0.107

HPRT 1

(0.202)

(0.149)

(0.169)

0.172

HSP90

(0.094)

(0.118)

(0.155)

0.122

PPI

(0.246)

(0.668)

(0.260)

0.391

SDH

(0.063)

(0.078)

(0.070)

0.070

TBP

(0.095)

(0.145)

(0.106)

0.115

YWHAZ

(0.197)

(0.038)

(0.029)

0.088

  1. aThe best gene has the lowest stability value