Table 4 Summarised results of the universal RNA standard spiked before transcription (part 1), obtained using CFX Manager software

E (90 to 110%)^a

98.9 to 126.3%

99.7 to 158.7%

82.2 to 89.1%

102.7 to 105.7%

91.3 to 94.7%

100 to 101.5%

104 to 127.7%

125.2 to 146.6%

99.7 to 101.2%

81.7 to 88.2%

R² (> 0.980)^b

0.973 to 0.996

0.834 to 0.996

0.955 to 0.997

0.960 to 0.993

0.981 to 0.999

0.987 to 0.997

0.969 to 0.992

0.910 to 0.996  

0.992 to 0.997

0.984  to 0.995

Slope (− 3.1 to − 3.6)^a

− 2.819 to − 3.348

− 2.422 to − 3.330

− 3.614 to − 3.842

− 3.193 to − 3.258

− 3.457 to − 3.507

− 3.286 to − 3.321

− 2.798 to −3.226 

− 2.551 to − 2.837

− 3.236 to − 3.329

− 3.642 to − 3.855

NTC C_q

35.85 to 38

N/A

N/A

N/A

N/A

N/A

N/A

N/A

N/A

N/A