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Table 5 Summarised Cq results for AuNP-interference assessment of the universal RNA standard spiked before transcription (part 1), by using CFX Manager™ software

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene C aq

18S

ACTB

GAPDH

GUSB

HPRT1

HSP90

PPI

SDH

TBP

YWHAZ

0%AuNP

13.81–14.90

17.24–18.02

17.36–17.71

23.40–24.02

21.98–23.07

19.53–19.90

18.15–18.73

23.39–23.65

24.48–25.22

21.06–21.24

25%AuNP

15.23–15.71

17.55–18.11

17.82–18.14

23.64–24.18

22.06–23.32

19.85– 20.43

18.55– 19.48

23.79–24.11

25.06–25.52

21.45– 21.94

50%AuNP

15.36–16.57

17.22– 18.53

17.34– 18.24

23.82–24.31

22.21–23.49

20.02– 20.62

19.24–20.66

23.59–24.11

24.50– 25.86

21.43–21.73

75%uNP

15.65–16.44

18.22– 18.64

17.50–17.96

23.74–24.36

21.88–23.38

19.56–20.32

19.22– 20.22

24.09– 24.28

24.69– 25.78

21.64– 21.87

  1. Bold italic acceptable result; italics unacceptable result; plain text base-line result, e.g. for untreated/0%AuNP samples
  2. aA Cq change of 0.2 is acceptable, but > 0.5 is unacceptable