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Table 5 Summarised Cq results for AuNP-interference assessment of the universal RNA standard spiked before transcription (part 1), by using CFX Manager™ software

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene C aq 18S ACTB GAPDH GUSB HPRT1 HSP90 PPI SDH TBP YWHAZ
0%AuNP 13.81–14.90 17.24–18.02 17.36–17.71 23.40–24.02 21.98–23.07 19.53–19.90 18.15–18.73 23.39–23.65 24.48–25.22 21.06–21.24
25%AuNP 15.2315.71 17.55–18.11 17.82–18.14 23.64–24.18 22.06–23.32 19.85– 20.43 18.55– 19.48 23.79–24.11 25.06–25.52 21.45– 21.94
50%AuNP 15.36–16.57 17.22– 18.53 17.34– 18.24 23.82–24.31 22.2123.49 20.02– 20.62 19.2420.66 23.59–24.11 24.50– 25.86 21.43–21.73
75%uNP 15.6516.44 18.22– 18.64 17.50–17.96 23.74–24.36 21.88–23.38 19.56–20.32 19.22– 20.22 24.09– 24.28 24.69– 25.78 21.64– 21.87
  1. Bold italic acceptable result; italics unacceptable result; plain text base-line result, e.g. for untreated/0%AuNP samples
  2. aA Cq change of 0.2 is acceptable, but > 0.5 is unacceptable