Table 7 Summarised results of the universal RNA standard spiked during amplification (part 2), as generated by the NormFinder analyses
Reference gene | NormFinder technical repeat 1 | NormFinder technical repeat 2 | NormFinder technical repeat 3 | NormFinder average | Manual ranking using the NormFinder average of the reference gene | NormFinder global summary of the best genea |
|---|---|---|---|---|---|---|
18S | (0.040) | (0.007) | (0.011) | 0.019 | 1 18S (most stable) 2 HPRT1 3 GUSB 4 YWHAZ 5 GAPDH 6 HSP90 7 ACTB 8 TBP 9 SDH 10 PPI (least stable) | GUSB (0.093) HPRT1 and YWHAZ (0.069) |
ACTB | (0.467) | (0.151) | (0) | 0.206 | ||
GAPDH | (0.074) | (0.007) | (0.185) | 0.088 | ||
GUSB | (0.014) | (0.021) | (0.070) | 0.035 | ||
HPRT1 | (0.078) | (0.017) | (0) | 0.031 | ||
HSP90 | (0.035) | (0.017) | (0.368) | 0.14 | ||
PPI | (0.999) | (0.893) | (0.519) | 0.803 | ||
SDH | (0.286) | (0.502) | (0.158) | 0.315 | ||
TBP | (0.069) | (0.214) | (0.415) | 0.232 | ||
YWHAZ | (0.074) | (0.024) | (0.114) | 0.070 |