Skip to main content

TableĀ 7 Summarised results of the universal RNA standard spiked during amplification (part 2), as generated by the NormFinder analyses

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene NormFinder technical repeat 1 NormFinder technical repeat 2 NormFinder technical repeat 3 NormFinder average Manual ranking using the NormFinder average of the reference gene NormFinder global summary of the best genea
18S (0.040) (0.007) (0.011) 0.019 1 18S (most stable)
2 HPRT1
3 GUSB
4 YWHAZ
5 GAPDH
6 HSP90
7 ACTB
8 TBP
9 SDH
10 PPI (least stable)
GUSB (0.093)
HPRT1 and YWHAZ (0.069)
ACTB (0.467) (0.151) (0) 0.206
GAPDH (0.074) (0.007) (0.185) 0.088
GUSB (0.014) (0.021) (0.070) 0.035
HPRT1 (0.078) (0.017) (0) 0.031
HSP90 (0.035) (0.017) (0.368) 0.14
PPI (0.999) (0.893) (0.519) 0.803
SDH (0.286) (0.502) (0.158) 0.315
TBP (0.069) (0.214) (0.415) 0.232
YWHAZ (0.074) (0.024) (0.114) 0.070
  1. aThe best gene has the lowest stability value