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Table 9 Summarised Cq results for AuNP-interference assessment of the universal RNA standard spiked at the amplification step (part 2), by using CFX Manager™ software

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene C aq

18S

ACTB

GAPDH

GUSB

HPRT1

HSP90

PPI

SDH

TBP

YWHAZ

0%AuNP

13.36–14.39

16.80–18.12

16.93–18.25

23.03–23.75

22.16–23.41

19.14–20.12

18.29–19.05

22.45–24.64

24.12–25.52

20.47–21.34

25%AuNP

14.20–14.95

17.67–18.15

17.44–18.69

23.66–24.36

23.10–24.30

19.33–20.37

19.97–21.04

22.89–24.22

24.86–25.50

21.15–21.73

  1. Due to restrictions on the final reaction volume per qPCR, only a 25% vol/vol AuNP spike could be accommodated
  2. Bold italics acceptable result; italics unacceptable result; plain text base-line result, e.g. for untreated/0%AuNP samples
  3. aA Cq change of 0.2 is acceptable, but > 0.5 is unacceptable