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Table 9 Summarised Cq results for AuNP-interference assessment of the universal RNA standard spiked at the amplification step (part 2), by using CFX Manager™ software

From: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Reference gene C aq 18S ACTB GAPDH GUSB HPRT1 HSP90 PPI SDH TBP YWHAZ
0%AuNP 13.36–14.39 16.80–18.12 16.93–18.25 23.03–23.75 22.16–23.41 19.14–20.12 18.29–19.05 22.45–24.64 24.12–25.52 20.47–21.34
25%AuNP 14.2014.95 17.6718.15 17.4418.69 23.6624.36 23.1024.30 19.3320.37 19.9721.04 22.8924.22 24.8625.50 21.1521.73
  1. Due to restrictions on the final reaction volume per qPCR, only a 25% vol/vol AuNP spike could be accommodated
  2. Bold italics acceptable result; italics unacceptable result; plain text base-line result, e.g. for untreated/0%AuNP samples
  3. aA Cq change of 0.2 is acceptable, but > 0.5 is unacceptable