Fig. 3From: Synthesis of europium-doped VSOP, customized enhancer solution and improved microscopy fluorescence methodology for unambiguous histological detectionStability of Eu fluorescence in presence of free iron(III) ions. The fluorescence intensity developed by each enhancer solution was set to 100% for a solution with constant cfinal(Eu) = 50 nmol/L and without iron(III). The fluorescence signal intensity developed by DELFIA was reduced to 10% when exposed to c(Fe) > 1 µmol/L while the Eu fluorescence intensity developed with HEE remained at 100% in the presence of c(Fe) up to 100 µmol/L. The SD of the mean fluorescence signal (n = 3) for HEE variates between 0.7 and 8% and between 0.3 and 11% for DELFIAⓇ Back to article page