Fig. 2
From: Ions doped melanin nanoparticle as a multiple imaging agent
Chelating capacity of the various ions onto MNPs by ICP-AES analysis (a). T 1-w MR (3.0-T, Philips) and CT (Inveon™, Siemens) contrast signal with different concentrations of ions (in iMNP) for sensitivity measurement (b). Protein quantification of antibodies conjugated onto the iMNP by BCA assay (c). Protein levels of various biomarkers concerned with cell organelle function or proliferation for measuring cytotoxicity after treatment by Western-blot analysis (d; i: only cell, ii: iMNP and iii: iMNP-EGFR; and see Additional file 1: Fig. S5)