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Table 1 High-throughput qRT-PCR of NHBE cells treated with CdSe or InP QDs

From: Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements

Cell response pathway Gene name Fold change (5 µg/mL, 24 h) Fold change (80 µg/mL, 6 h)
CdSe-MUA InP-MUA CdSe-MUA InP-MUA
DNA damage CDK1 0.50 ± 0.15 0.21 ± 0.09 1.92 ± 0.10 1.15 ± 0.01
GADD45A 1.18 ± 0.02 1.00 ± 0.09 4.17 ± 0.95 4.95 ± 0.66
SFN 1.50 ± 0.04 1.75 ± 0.04 2.84 ± 0.50 0.86 ± 0.44
DNA repair BRCA1 0.55 ± 0.07 0.18 ± 0.03 1.20 ± 0.05 0.96 ± 0.22
BRCA2 0.57 ± 0.07 0.20 ± 0.02 3.06 ± 0.48 0.43 ± 0.17
XPC 0.89 ± 0.02 0.79 ± 0.05 0.84 ± 0.15 2.29 ± 0.15
Mitochondrial function and repair AHR 0.99 ± 0.04 1.11 ± 0.05 2.18 ± 0.29 2.35 ± 0.43
CYP1A1 1.02 ± 0.11 1.17 ± 0.17 1.38 ± 0.35 0.17 ± 0.04
CYP1B1 1.29 ± 0.02 1.16 ± 0.07 3.19 ± 0.46 0.70 ± 0.09
DHFR 0.57 ± 0.02 0.40 ± 0.02 1.10 ± 0.10 1.15 ± 0.07
UCP1 2.29 ± 0.50 2.20 ± 1.64 4.32 ± 0.87 7.20 ± 0.51
Proliferation VEGFA 1.43 ± 0.05 1.44 ± 0.17 2.18 ± 0.48 1.21 ± 0.16
  1. Cells were incubated with 5 μg/mL CdSe or InP QDs for 24 h or with 80 μg/mL CdSe or InP QDs for 6 h. Gene expression changes for different classes of cellular functions and processes were measured by qRT-PCR. Values are expressed as fold change relative to media controls (= 1.0). Italics values indicates a twofold or greater decrease in gene expression relative to untreated cells (≤ 0.5) while bold italics highlights twofold or greater increases in gene expression relative to untreated cells (≥ 2.0). All experiments were independently repeated three times (n = 3). Errors represent one standard deviation